Strange Tales #110 (1963)
CGC 6.5 Serial # 4044070001
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1st appearance of Doctor Strange
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1st appearance of the Ancient One, former Sorcerer Supreme
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1st appearance of Wong, Doctor Strange's assistant
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[May 2022 - Wong to appear in She-Hulk Disney+ series]
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1st cameo appearance of Nightmare, multiple panels, named but obscured by shadows